WebOct 12, 2024 · The following day we added 300 μl of the Agrobacterium culture to 20 ml TY (pH 5.5) containing the appropriate antibiotics and 200 μM acetosyringone. We incubated the culture 28 °C for in a shaking incubator set at 250 rpm until the culture reached an OD 600 between 0.1 – 0.2 (approximately 2–4 h). WebAug 16, 2024 · Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome …
Highly efficient, genotype-independent transformation …
WebDec 4, 2024 · Efficient genotype-independent genetic transformation in watermelon by overexpressing a chimeric ClGRF4-GIF1 gene (A) Schematic diagram of the vectors … WebHighly efficient, genotype‐independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4‐GIF1 gene Author: Qin Feng, Ling Xiao, … peabody carpet cleaning
Karlee Grey Glasses - Vanilla Celebrity
WebOct 12, 2024 · a, Schematic representation of the GRF4 (blue) and GIF1 (pink) chimera. The black region represents a sequence encoding a four-amino-acid spacer. b, The GRF4–GIF1 transgenic wheat plants were ... WebDec 18, 2024 · Similarly, the GRF4-GIF1 chimeras from Citrus and grape (Vitis vinifera), respectively, enhanced the regeneration ability of dicotyledonous citrus. Mutations in the miR396 target site further elevated the activity of the grape GRF4-GIF1 chimera. The superior performance in both monocots and dicots highlights the broad application … WebChromosome segregation must be under strict regulation to maintain chromosome euploidy and stability. Cell Division Cycle 20 (CDC20) is an essential cell cycle regulator that promotes the metaphase-to-anaphase transition and functions in the spindle assembly checkpoint, a surveillance pathway that ensures the fidelity of chromosome segregation. scythe\\u0027s ld