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Clgrf4-gif1

WebOct 12, 2024 · The following day we added 300 μl of the Agrobacterium culture to 20 ml TY (pH 5.5) containing the appropriate antibiotics and 200 μM acetosyringone. We incubated the culture 28 °C for in a shaking incubator set at 250 rpm until the culture reached an OD 600 between 0.1 – 0.2 (approximately 2–4 h). WebAug 16, 2024 · Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome …

Highly efficient, genotype-independent transformation …

WebDec 4, 2024 · Efficient genotype-independent genetic transformation in watermelon by overexpressing a chimeric ClGRF4-GIF1 gene (A) Schematic diagram of the vectors … WebHighly efficient, genotype‐independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4‐GIF1 gene Author: Qin Feng, Ling Xiao, … peabody carpet cleaning https://jshefferlaw.com

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WebOct 12, 2024 · a, Schematic representation of the GRF4 (blue) and GIF1 (pink) chimera. The black region represents a sequence encoding a four-amino-acid spacer. b, The GRF4–GIF1 transgenic wheat plants were ... WebDec 18, 2024 · Similarly, the GRF4-GIF1 chimeras from Citrus and grape (Vitis vinifera), respectively, enhanced the regeneration ability of dicotyledonous citrus. Mutations in the miR396 target site further elevated the activity of the grape GRF4-GIF1 chimera. The superior performance in both monocots and dicots highlights the broad application … WebChromosome segregation must be under strict regulation to maintain chromosome euploidy and stability. Cell Division Cycle 20 (CDC20) is an essential cell cycle regulator that promotes the metaphase-to-anaphase transition and functions in the spindle assembly checkpoint, a surveillance pathway that ensures the fidelity of chromosome segregation. scythe\\u0027s ld

A GRF–GIF chimeric protein improves the regeneration ... - Nature

Category:Study on the optimization of transformation systems in watermelon

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Clgrf4-gif1

Journal: Journal of integrative plant biology / Publication year rev ...

WebJan 24, 2024 · Watch and share Clra4hbxroc41 GIFs on Gfycat. Search millions of user-generated GIFs WebHighly efficient, genotype‐independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4‐GIF1 gene Author: Qin Feng; Ling Xiao; Yizhen He; Man Liu; Jiafa Wang; Shujuan Tian; Xian Zhang; Li Yuan

Clgrf4-gif1

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WebDec 15, 2024 · Recent studies have shown that constitutive expression of either WOX5 or a GROWTH REGULATING FACTOR4 (GRF4) and GRF-INTERACTING FACTOR1 (GIF1) chimaeric protein enhances transformation efficiency... WebNov 9, 2024 · Feng Q, Xiao L, He Y, Liu M, Wang J, Tian S et al (2024) Highly efficient, genotype-independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4-GIF1 gene. J Integr Plant Biol 63(12):2038–2042. CrossRef CAS Google Scholar

WebExplore and share the best Fourth Grade Classroom GIFs and most popular animated GIFs here on GIPHY. Find Funny GIFs, Cute GIFs, Reaction GIFs and more. WebOct 22, 2024 · 4 Answers. Sorted by: 121. Yes, you need to wrap in quotes: "some value over two lines",some other value. From this document, which is the generally-accepted …

WebMar 1, 2024 · Similarly, the GRF4-GIF1 chimeras from Citrus and grape (Vitis vinifera), respectively, enhanced the regeneration ability of dicotyledonous citrus. Mutations in the miR396 target site further elevated the activity of the grape GRF4-GIF1 chimera. The superior performance in both monocots and dicots highlights the broad application … WebUse Read by QxMD to access full text via your institution or open access sources. Read also provides personalized recommendations to keep you up to date in your field.

WebClGRF4-GIF1 can also be combined with clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing tools to achieve …

WebGRF4-GIF1 chimera. A) Schematic representation of the GRF4 (blue)-GIF1 (pink) chimera. The black region represents a four amino acid spacer. B) The GRF4-GIF1 transgenic wheat plants were normal and fertile.C) Representative transformation showing higher frequency of regenerated shoots during Kronos transformation in the presence of the GRF4-GIF1 … peabody carpet cleaning san joseWebDec 4, 2024 · Chimeric ClGRF4-GIF1 can also be combined with CRISPR/Cas9 genome editing tool to achieve highly efficient gene editing in watermelon, by which we … peabody career centerWebM Liu, DQ Shi, L Yuan, J Liu, WC Yang. Journal of Integrative Plant Biology 52 (9), 817-828. , 2010. 51. 2010. Arabidopsis DAYU/ABERRANT PEROXISOME MORPHOLOGY9 Is a Key Regulator of Peroxisome Biogenesis and Plays Critical Roles during Pollen Maturation …. XR Li, HJ Li, L Yuan, M Liu, DQ Shi, J Liu, WC Yang. scythe\\u0027s lbWebHighly efficient, genotype‐independent transformation and gene editing in watermelon ( Citrullus lanatus ) using a chimeric ClGRF4‐GIF1 gene scythe\\u0027s lhWebQin Feng, Ling Xiao, Yizhen He, Man Liu, Jiafa Wang, Shujuan Tian, Xian Zhang, and Li Yuan. "Highly efficient, genotype‐independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4‐GIF1 gene" Journal of integrative plant biology 63, no. 12 (2024): 2038-2042. doi: 10.1111/jipb.13199 scythe\u0027s leWebGRF4-GIF1 transgenic plants were fertile and without obvious developmental defects. Moreover, GRF4-GIF1 induced efficient wheat regeneration in the absence of exogenous … scythe\u0027s loWebDec 1, 2024 · By overexpressing a chimeric fusion of ClGRF4 and ClGIF1, we achieved highly efficient transformation in watermelon. Mutating the mi396 microRNA target site in ClGRF further boosted the transformation efficiency up to 67.27% in a genotype-independent manner. peabody career achievement award