Pcdna3.1 myc his a

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August undefined, 2024

SpletSuperCos I pYr-adshuttle-3 pVitro2-neo-mcs pUB6/V5-His /LacZ pUB6/V5-His C pUB6/V5-His B pUB6/V5-His A pTracer-CMV2 pSV-β-Galactosidase pSI pSG5 pSFV1 … SpletPcdna3 1 Flag Env38 Ltr, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

pCDNA3.1-3×Myc-HNRNPF(human)-质粒载体-ATCC-DSM-CCUG

SpletpcDNA3.1MycHisA (Invitrogen). pTRE-DRAM-Myc/His was generated through digestion of pcDNA3-DRAM-Myc/His with HindIII and Pme1 followed by cloning in to the HindIII and EcoRV sites of pTRE2 (Clontech). Luciferase constructs p53BS-1 luc and p53BS-2 luc were generated using regions within DRAM Intron 1 using the Splet01. sep. 2011 · The pcDNA3.1-Flag-ERRα and pcDNA-myc/his-PGC-1α have been described previously . Transient transfection in C2C12 myocytes by the calcium phosphate method has been described previously . Briefly, subconfluent C2C12 myoblasts were plated in 24-well plates at a density of 13,000 cells/well 1 day before transfection. fiche technique primer t

Cilium-independent regulation of Gli protein function by Sufu in ...

SpletpGenDONR. If your experiments require custom DNA constructs in one of these expression vectors, then GenScript's Express Cloning service (SC1691) is your best option – with an … http://formedium.equl.cn/category.php?id=386 http://www.biofeng.com/zaiti/buru/pcDNA3.1-Myc-HisB.html fiche technique thermo loft

DELTEX2 C-terminal domain recognizes and recruits ADP …

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SpletInvitrogen™ pcDNA™3.1/. myc. -His A, B, & C Mammalian Expression Vectors. Contain a strong promoter and a selection marker for generating stable cell lines. Supplier: … Splet哺乳表达质粒 pCDNA3.1(+)-3HA. 哺乳表达质粒 pCDNA3.1-Myc-HisB. 哺乳表达质粒环状RNA表达 pcDNA3.1(+) CircRNA Mini Vector（addgene 60648）哺乳表达质粒 pcDNA6 … fiche technique thermosan caparolSplet累死人不偿命后来我自己对着图谱研究了一下 (我所使用的是 pCDNA3.0 载体、 pCDNA3.1 myc his C 载体和 pEGFP 载体) .0 的 MCS 两端有 T7 和 SP6 如果我用 T7 和 SP6 作为引物,则 1.P 出大小约 150bp 的片段,则为 ... fiche technique starkey evolv ai 2400 iic nw

"SpletHEK293 cells were seeded in a 24-well plate to obtain around 70 % confluence at the day of transfection. A total of 250 ng of either wt or variant SOX4-Large BiT-expressing NanoBiT … " - Pcdna3.1 myc his a

Pcdna3.1 myc his a

SpletInvitrogen pcDNA3.1(-)/myc-His A 哺乳动物表达载体 HG-VPI0017 -上海易扩仪器有限公司_Equl意果_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington Splet13. feb. 2015 · Immunoprecipitation of V5-3 C-MYC-tagged wild-type Tb14-3-3 (W) ... About 1.6 µg of His-AKB1, 1 µg of recombinant Hu-calmodulin, and recombinant His-Tb-calmodulin, 1 mM ATP and 1 mM calcium chloride were used for the Pep-Tag kinase assay. – and + show in the absence and in the presence of the indicated factors in the kinase …

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SpletpcDNA™3.1 (-) 哺乳动物表达载体 Catalog number: V79520 11,170.00 / 20 µg 添加至购物车 pcDNA™3.1/ myc -His A、B & C 哺乳动物表达载体 Catalog number: V80020 13,629.00 / … SpletThe gene coding c-Myc was amplified from the cDNA of U87 cells, and the product was cloned into pcDNA3.1 vector. Finally, the control and c-Myc plasmids were transfected into U87 KD cells. Isolation of RNA and Quantitative Real-Time PCR

SpletpcDNA3.1/Myc-His A载体价格1000元，pcDNA3.1/Myc-HisA载体质粒图谱(Vector map),载体序列(Sequence)和多克隆位点见下文，载体抗性为氨苄，质粒大小为5494 bp，测序引 … Splet质粒载体网zlzt.com专业提供pCDNA3.1-MMP9-His-Myc,产品编号为zl-023159,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏!

SpletpcDNA3.1(-)/myc-His A PVTY00939 2ug Alias:pcDNA3.1/myc-His(-) APlasmid type:Mammalian expression vectorPromoter:CMVCloning Method:Multiple cloning … http://users.auth.gr/pchristo/research/plasmids/com/pcDNA%206_His%20A,B,C.pdf

SpletTo propagate and maintain the pcDNA6/His vectors, we recommend resuspending each vector in 20 µl sterile water to prepare a 1 µg/µl stock solution. Store the stock solution at …

Splet21. avg. 2024 · Mammalian constructs were cloned into pcDNA3 modified with an N-terminal Myc-tag (pcDNA3Myc), pGZdx21Z , pcDNA3.1 B(+), or mCherry2-C1, a gift from M. Davidson (Addgene plasmid no. 54563). Protein expression and purification ... Crystals of form 1 (His-2RD) and form 2 (2RD) were obtained in 0.1 M tris-HCl (pH 7.6), 36% glycerol … fiche technique thermopompe nirvanaSpletA guide to Addgene's empty vector backbones curated by species, epitope tags/fusion protein, choosing marker, viral expression, and more. fiche technique thermor malicio 3http://www.honorgene.com/product_list/carrier_library/123248.html fiche technique thermofibre gutexSpletAs of April 1, 2024, we increased some of our prices. See the new prices and get more information or speak with our friendly support team . Close Search Log In Create Account … grenadines official languageSplet13. okt. 2024 · pcDNA3.1(+) 和 pcDNA3.1(-) 是源自 pcDNA3 的 5.4 kb 载体，设计用于在哺乳动物宿主中进行高水平的稳定和瞬时表达。可以在大多数哺乳动物细胞中进行高水平 … grenadine south africaSplet14. apr. 2024 · The pCMV-Myc-STAT3 was cloned into mammalian expression pCDNA3-Myc vectors. pGEX-4T1-SIK1 were cloned into mammalian expression pGEX-4T1 vectors. pLenti-puro-SIK1 was cloned into mammalian expression pLenti-puro vectors. SIK1-P187A/P188A/Y189A, SIK1-S65A, SIK1-S435A, SIK1-S435D, AKT1-E17K, Pin1-V34A/K63A, … fiche technique tomate pdfSplet21. jul. 2024 · High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His (-)/lacZ, is the … grenadines palm island resort