Phis2.1

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August undefined, 2024

Webb1 site: C C C A G C G G G T C G: After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. AmpR 1973 .. 2833 = 861 bp 286 amino acids = 31.6 kDa 2 segments WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1 bait plasmid. phis2 1 bait plasmid TaKaRa is a ...

Transcription Factor-Centered Yeast One-Hybrid Assay

Webb1 dec. 2024 · According to the hereditary mode of sterility genes, this vital agronomic trait can be further divided into cytoplasmic male sterility (CMS) and genic male sterility … http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf bitcoin reliability

Phis2 TaKaRa Bioz

Webb17 juni 2024 · Expression pattern analysis of OsbZIP62 under abiotic stresses and hormones. a, Relative expression level of OsbZIP62 under various stresses, including PEG6000 (15%), cold (4 °C), heat (42 °C), NaCl (150 mM), H 2 O 2 (1%) and ABA (100 μM). b, Main stress-related cis-acting elements in the OsbZIP62 promoter region.c, Relative … WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webbyeast reporter vector phis2 1 bd ( TaKaRa ) 95. TaKaRa yeast reporter vector phis2 1 bd. Yeast Reporter Vector Phis2 1 Bd, supplied by TaKaRa, used in various techniques. Bioz … dash and lily online subtitrat

Functional dissection of a strong and specific microbe‐associated ...

PT3705-5 (PR32049) pHIS2

Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors. WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … dash and lily musicWebb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . bitcoin research

"Webb5 feb. 2024 · Either pGADT7-RrMYB18 or pGADT7-AtMYB46 was used to co-transform yeast strain Y187 with either the pHIS2.1-P RrCESAs or pHIS2.1-P AtCYCs reporter plasmids, respectively. DNA–protein interactions were quantified using the Matchmaker one-hybrid system (Clontech, now TaKaRa) according to the manufacturer’s instructions … " - Phis2.1

Phis2.1

Webb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls. Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional …

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Webb25 okt. 2016 · The 43-bp NWRE fragment amplified by PCR was inserted into pHIS2.1 (Clontech, CA, USA) to generate the plasmid pHIS2.1-NWRE as bait. The coding regions of IbbHLH3 and IbbHLH4 were separately cloned into pGADT7 as effectors. Lithium acetate-mediated transformation was performed to transform the constructs into yeast strain … Webb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ...

Webb27 mars 2015 · In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. As prey to select for TFs that bind to the … Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained …

Webb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 … WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: …

WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … bitcoin rendite rechnerWebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … das handling servicesWebb+ 5 µg pHis2.1 20 µl Note: To denature carrier DNA, heat to 95–100°C for 5 min, then cool rapidly in an ice bath. NOTE NOTE Protocol No. PT3529-1 Version No. PR7Z2445 32 3. Add competent cells and gently mix. 4. Add PEG/LiAc and gently mix. 5. Incubate at 30°C . NOTE: Mix cells every 10–15 min. www.clontech.com 50 µl 500 µl 30 min 600 µl 2.5 ml dash and lily free onlineWebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is … bitcoin research topicsWebb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. das handelingsindicatorenWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … bitcoin research paperWebb18 okt. 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). bitcoin revolution auto trading review